Protein Purification
Hampton Research HR2-996-48/Silver Bullets #48 (D12), 0.25 ml
HR2-996-48/Silver Bullets #48 (D12), 0.25 ml. Please note items from this supplier are non-returnable.
Abcam Nuclear E x traction Kit, 100 Tests
This Nuclear Extraction Kit is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in our transcription factor assay kits.
The product is subject to the following: Abcam Restricted Use Statement
STEMCELL Technologies XAV939, Size: 25 mg
Inhibit tankyrase (TNKS) 1 and 2 with XAV939 (Huang et al. Nature, 2009). By inhibiting TNKS activity, XAV939 increases the protein levels of the axin-GSK3β complex and promotes the degradation of β-catenin in SW480 cells (Huang et al. Nature, 2009). This WNT pathway inhibitor inhibits colony formation of APC-deficient, β-catenin-dependent DLD-1 colorectal cancer cells (Huang et al.); induces cardiomyogenesis in mesoderm progenitor cells derived from mouse embryonic stem cells (Wang et al.); and, in combination with the SMAD inhibitors LDN193189 and SB431542, promotes induction of forebrain fates in human pluripotent stem cell lines (Maroof et al.). XAV939 is supplied as a crystalline solid with ≥ 98% purity; Molecular weight 312.3 g/mol; CAS number 284028-89-3. Visit STEMCELL.com for more information and related products.
LifeSensors Tandem Ubiquitin Binding Entities (TUBE1) HC Magnetic 1mL
Tandem Ubiquitin Binding Entities (TUBEs) are engineered proteins with strong affinity for ubiquitin, showing up to a 1000‑fold increase in binding to polyubiquitin chains compared to single UBAs. They protect polyubiquitinated proteins from degradation, enabling sensitive detection and efficient isolation of native ubiquitinated proteins from cell and tissue extracts. TUBEs “capture” proteins in their polyubiquitinated state, making them valuable for enrichment and identification studies. Magnetic TUBE 1 beads allow rapid, single‑step recovery of polyubiquitinated proteins without centrifugation, improving removal of unbound material and reducing background. Proteins can be easily eluted for downstream Western blot or proteomic analysis. UM501M uses high‑capacity polymeric magnetic beads to enhance enrichment while minimizing nonspecific binding.
ACROBiosystems This product is intended for immunocapture, biopanning and flow cytometry.
The Human TROP-2-coupled Magnetic Beads is produced by coupling biotinylated TROP-2 heterodimer to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-TROP-2 antibody from your sample with high specificity.
STEMCELL Technologies Cyclic Pifithrin-Alpha (Hydrobromide), Size: 10 mg
Cyclic Pifthrin-Alpha is a cell-permeable and reversible inhibitor of p53-mediated apoptosis and p53-dependent gene transcription. It is a more stable and less cytotoxic analog of the non-cyclic form of pifithrin-alpha. Cyclic Pifithrin-Alpha has also been reported to activate the aryl hydrocarbon receptor. (Fernandez-Cruz et al. Gary Jensen Komarov et al.)
STEMCELL Technologies EasySep™ Human CD8 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD8 Positive Selection Kit II is designed to isolate CD8+ cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection. Desired cells are targeted with antibody complexes recognizing CD8 and magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD8 antigen is expressed on cytotoxic T cells and weakly on a subset of NK cells.This product replaces the EasySep™ Human CD8 Positive Selection Kit (Catalog #18053) for even faster cell isolations.
Supply Solutions Sigma Aldrich - Complete Edta-Free 3 X 20 Tablets * Third Party Item
Sigma Aldrich - Complete Edta-Free 3 X 20 Tablets * Third Party Item
STEMCELL Technologies EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit, For processing: 1 x 10^9 cells
This kit isolates human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. First, CD25+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells, and unwanted non-Tregs are targeted for depletion. The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications. An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.